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KMID : 0545120070170122081
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 12 p.2081 ~ p.2084
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Expression of α-Galactosidase Gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum
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Park Jae-Yong
Jeong Seon-Ju
Lee Ae-Ran
Park Ji-Yeong
Jeong Woo-Ju
Kim Jeong-Hwan
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Abstract
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A 2.5 kb aga gene encoding α-galactosidase (α- Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in cells grown on raffinose (1%, w/v) followed by cells grown on galactose, melibiose=fructose, glucose, and sucrose. Western blot using antibodies against α- Gal showed similar results to slot-blot results and enzyme activity measurements. All the results indicated that the heterologous aga was successfully expressed in L. citreum and its transcription was under the carbon catabolite repression (CCR).
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KEYWORD
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α-Galactosidase, gene expression, Leuconostoc citreum, CCR
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